Argyris Politis Research Group

Publications

  • Charlotte Guffick,Argyris Politis.2025, "HDX-MS in micelles and membranes for small molecule and biopharmaceutical development", Current Opinion in Structural Biology, Volume 94, October 2025, 103077 [doi].
  • Vanessa Duerr, Damon Griffiths,Argyris Politis.2025, "MultiPassMerger: Automated data processing for multipass cyclic ion mobility HDX-MS", Protein Science, 24 May 2025 [doi].
  • Rincon Pabon JP, Akbar Z, Politis A. MSe Collision Energy Optimization for the Analysis of Membrane Proteins Using HDX-cIMS, Journal of the American Society for Mass Spectrometry, 3;(35(7):1383-89, 2024, doi:10.1021/jasms.4c00093
  • Toporowska J,Kapoor P, Musgarrad M, Gherbi K, Sengmany K, Qu F, Yen HY, Hansen K, Jazayeri A, Hopper JTS, Politis A*. Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen deuterium exchange mass spectrometry. Nature Communications, 15, 8993, 2024, doi:10.1038/s41467-024-53161-0
  • Griffiths D, Anderson M, Richardson K, Inaba-Inoue S, Allen WJ, Collinson I, Beis K, Morris M, Giles K, Politis A*. Cyclic ion mobility for hydrogen/deuterium exchange mass spectrometry applications. Analytical Chemistry, 2024, 96(15): 5869-5877
  • Javed W, Griffiths D, Politis A*. Hydrogen/deuterium exchange-mass spectrometry of integral membrane proteins in native-like environments: current scenario and the way forward. Essays in Biochemistry, 67(2): 187-200, 2023.
  • Jia R, Bradshaw R, Calvaresi V, Politis A*. Integrating Hydrogen Deuterium Exchange–Mass Spectrometry with Molecular Simulations Enables Quantification of the Conformational Populations of the Sugar Transporter XylE. Journal of the American Chemical Society, 145, 14, 7768-79, 2023.
  • Calvaresi V, Wrobel AG, Toporowska J, Hammerschmid DH, Doores KJ, Bradshaw RT, Parsons RB, Benton DJ, Roustan C, Reading E, Malim MH, Gamblin SJ, Politis A*. Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein. Nature Communications 14,1421,
  • Yen HY, Liko I, Song W, Kapoor P, Almeida F, Toporowska J, Gherbi K, Hopper JTS, Charlton SJ, Politis A, Sansom MSP, Jazayeri A, Robinson CV. Mass Spectrometry Captures Biased Signalling and Allosteric modulation of a G-protein-coupled receptor. Nature Chemistry, 14,12, 1375-82,

  • Hammerschmid D, Calvaresi V, Bailey C, Russel LB, Politis A, Morris M, Denbigh L, Anderson M, Reading E. Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein-Lipid Assemblies. Analytical Chemistry 95(5):30002-4, 2023
  • Seow J, Khan H, Rosa A, Calvaresi V, Graham C, Pickering S, Pye VE, Cronin NB, Huettner I, Malim MH, Politis A*, Cherepanov P*, Doores KJ*. A Neutralizing Epitope on the SD1 Domain of SARS-CoV-2 spike Targeted Following Infection and Vaccination, Cell Reports, 40, 8, 111276,
  • Norgate EL, Upton R, Hansen K, Bellina B, Brookes C, Politis A, Barran PE. Cold denaturation of proteins in the absence of solvent: Implications for protein storage. Angewandte Chemie, 2021;15047, 2022.
  • Ciftci D, Martens C, Ghavi Cg, Blanchard SC, Politis A, Huysmans GHM, Boudker O. Linking function to global and local dynamics in an elevator-type transporter. Proceedings of the National Academy of Sciences of the USA, 118(49):e2025520118,
  • Lau AM, Politis A*. Integrative Mass Spectrometry Based approaches for modelling macromolecular complexes. Methods in Molecular Biology, 2020, 2247:221-41, 2021.
  • Jia R, Martens C, Shekhar M, Pant S, Pellowe GA, Lau AM, Findlay HE, Harris NJ, Tajkhorshid, Booth PJ, Politis A*. Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter. Nature Communications, 11: 6162, 2020.
  • Martens C, Politis A*. A glimpse into the molecular mechanism of integral membrane proteins through hydrogen-deuterium exchange mass spectrometry. Protein Science, 29(6):1285-1301. 2020.
  • Reading E, Ahdash Z, Fias C, Ricci V, Wang kan X, Grimsey E, Stone J, Malloci G, Lau AM, Findlay H, Konijnenberg A, Booth PJ, Ruggerone P, Vargiu AV, Piddock LJV, Politis A*. Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB. Nature Communications, 11(1): 5565, 2020.
  • Lau AM, Jia R, Bradshaw RT, Politis A*. Structural predictions of the functions of membrane proteins by HDX-MS. Biochemical Society Transactions, 48(3):971-979, 2020.
  • Stutzer A, Welp LM, Raabe M, Sachsenberg T, Kappert C, Wulf A, Lau AM, David SS, Chernev A. Kramer K, Politis A, Kohlbacher O, Fischle W, Urlaub H. Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry. Nature Communications, 11(1): 5250, 2020.
  • Zhang T, Hansen K, Politis A, Muller MM. An unusually rapid protein backbone modification stabilizes the essential bacterial enzyme MurA. Biochemistry. 59(39):3683-3695, 2020.
  • Lau AM, Claesen J, Hansen K, Politis A*. Deuteros 2.0: peptide-level significance testing of data from hydrogen deuterium exchange mass spectrometry. Bioinformatics, 37(2):270-272, 2021.
  • Allison TM, Barran P, Cianférani S, Degiacomi MT, Gabelica V, Grandori R, Marklund EG, Menneteau T, Migas LG, Politis A, Sharon M, Sobott F, Thalassinos K, Benesch JLP. Computational strategies and challenges for using native ion mobility mass spectrometry in biophysics and structural biology. Analytical Chemistry, 92(16):10872-10880, 2020.
  • Allison TM, Barran P, Benesch JLP, Cianferani S, Degiacomi MT, Gabelica V, Grandori R, Marklund EG, Menneteau T, Migas LG, Politis A, Sharon M, Sobott F, Thalassinos K. Software requirements for the analysis and interpretation of native ion mobility mass spectrometry data. Analytical Chemistry, 92(16):10881-10890.
  • Hansen KJ, Politis A*. Improving Peptide Fragmentation for Hydrogen-Deuterium Exchange Mass Spectrometry Using a Time-Dependent Collision Energy Calculator. Journal of the American Society for Mass Spectrometry, 31, 4, 996-9, 2020.
  • Kourkoulou A, Grevias P, Lambrinidis G, Pyle E, Dionysopoulou M, Politis A, Mikros E, Byrne B, Diallinas G. Specific residues in a purine transporter are critical for dimerization, ER-exit and function. Genetics, 213(4):1357-1372, 2019.
  • Martens, C.; Shekhar, M.; Lau, A.M.; Tajkhorshid, E., Politis, A*. Integrating hydrogen-deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins. Nature Protocols, 14, 3183–3204,
  • Pyle E, Guo C, Hofmann T, Schmidt C, Ribiero O, Politis A*, Byrne B*. Protein-Lipid Interactions Stabilize the Oligomeric State of BOR1p from Saccharomyces cerevisiae. Analytical Chemistry, 91:20, 13071-13079, 2019.
  • Faull SV, Lau AM, Martens C, Ahdash Z, Yebenes H, Schmidt C, Beuron F, Cronin NB, Morris EP, Politis A*. Structural Basis of Cullin-2 Ring E3 Ligase by the Cop9 Signalosome. Nature Communications 10:3814, 2019.
  • Ahdash Z, Pyle E, Allen WJ, Corey RA, Collinson I, Politis A* HDX-MS reveals nucleotide-dependent, anti- correlated opening and closure of SecA and SecY channels of the bacterial translocon. eLife 8:e47402, 2019.
  • Lau AM, Ahdash Z, Martens C, Politis A* Deuteros: software for rapid analysis and visualization of data from differential HDX-MS. Bioinformatics, 35(17): 3171-3173, 2019.
  • Corey RA, Ahdash Z, Shah A, Pyle E, Allen WJ, Lovett JE, Politis A*, Collinson I. ATP-induced assymetric pre- protein folding as a driver of protein translocation through the Sec machinery. eLife, e41803, 2019.
  • Hellwig N, Peetz O, Ahdash Z, Tascon I, Booth PJ, Mikusevic V, Diskowski M, Politis A, Hellmich Y, Hanelt I, Reading E and Morgner N. Native MS goes more native: investigation of membrane protein complexes directly from SMALPs. Chemical Communications 54 13702-5, 2018.
  • Hansen KJ, Lau AM, Giles K, McDonnell J, Sutton B, Politis A*. A mass spectrometry-based modelling workflow for accurate prediction of IgG antibody conformations in the gas phase. Angewandte Chemie 57, 1-7, 2018.
  • Ahdash Z, Lau AM, Martens C, Politis A*. Analyzing protein architectures and protein-ligand complexes by integrative structural mass spectrometry. Journal of Visualised Experiments (140), e57966, 2018.
  • Claesen J, Politis A* POPPeT: a new method to predict the protection factor of backbone amide hydrogens. Journal of the American Society for Mass Spectrometry,30:1, 67-76, 2019.
  • Pernigo S, Chegkazi MS, Yip YY, Treacy C, Glorani G, Hansen K, Politis A, Bui S, Dodding MP, Steiner RA. Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors. eLife, 7, e38362, 2018.
  • Martens C, Shekhar M, Borysik AJ, Lau AM, Reading E, Tajkorshid E, Booth PJ, Politis A*. Direct protein-lipid interactions shape the conformational landscape of secondary transporters. Nature Communications 9;4151, 2018.
  • Corey, RA, Pyle E, Allen WJ, Watkins DW, Casiraghi M, Miroux B, Arechaga I, Politis A, Collinson I. Specific cardiolipin-SecY interactions are required for proton-motive force stimulation of protein secretion. Proceedings of the National Academy of Sciences of the USA 115 (31) 7967-72, 2018.
  • Politis A*, Schmidt C. Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry and computational modelling. Journal of Proteomics. 20;175, 34-41, 2018.
  • Pyle E, Kalli AC, Amillis S, Hall Z, Lau AM, Hanyaloglu AC, Diallinas G, Byrne B, Politis A*. Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA. Cell Chemical Biology 25 7, 840-8, 2018.
  • Haupt C, Hofmann T, Wittig S, Kostmann S, Politis A, Schmidt C. Combining chemical cross-linking and mass spectrometry of intact protein complexes to study the architecture of multi-protein assemblies. Journal of Visualised Experiments (129),56747 2017.
  • Ahdash Z, Lau AM, Byrne RT, Lammens K, Stuetzer A, Urlaub H, Booth PJ, Reading E, Hopfner KP, Politis A*. Mechanistic insight into the assembly of the HerA-NurA helicase-nuclease DNA end resection complex Nucleic Acids Research 45;20, 12025-38, 2017.
  • Reading E, Hall Z, Martens C, Haghighi T, Findlay H, Ahdash Z, Politis A, Booth PJ. Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions. Angewandte Chemie 56, 15654-15657, 2017.
  • Schmidt C, Mcpherson JA, Lau AM, Tan KW, Fraternali F, Politis A* Surface Accessibility and Dynamics of Macromolecular Assemblies Probed by Covalent Labeling MS and Integrative Modeling. Analytical Chemistry 89, 1459-68, 2017.
  • Hall Z, Schmidt C, Politis A*. Uncovering the early assembly mechanism for amyloidogenic β2 microglobulin using cross-linking and native mass spectrometry. Journal of Biological Chemistry, 291, 4626-4637, 2016.
  • Politis A*, Borysik, A. Assembling the of macromolecular complexes: A hybrid structural biology approach, Proteomics, 15:16. 2792-803, 2015.
  • Ahdash Z, Pyle E, Politis A* Hybrid MS: Towards characterization of protein conformational states. Trends in Biochemical Sciences, 41:8, 650-53, 2016 
  • Politis A, Schmidt C, Tjioe E, Lasker K, Gordiyenko Y, Russell D, Sali A, Robinson CV. Topological models of heteromeric protein assemblies from mass spectrometry: Application to the yeast eIF3:eIF5 complex. Chemistry & Biology 22:1, 117-28, 2015.
  • Marcoux J, Polits A, Rinehart D, Marshall DP, Wallace MI, Tamm LK, Robinson CV. Mass spectrometry defines the C-terminal dimerization domain and enables modeling of the structure of full-length OmpA. Structure 22:5, 781-90, 2014.
  • Zhou M#, Politis A#, Davies R, Liko I, Stewart A, Stock D, Robinson CV. Ion mobilit-mass spectrometry of a rotary ATPase reveals ATP-induced reduction in conformational flexibility. Nature Chemistry 6:3, 208-15, 2014.
  • Politis A, Stengel F, Hall Z, Hernandez H, Leitner A, Waltzhoeni T, Robinson CV, Aebersold R. A mass spectrometry-based hybrid method for structural modelling of protein complexes. Nature Methods 11:4, 403-6,
  • Rouillon C, Zhou M, Zhang J, Politis A, Beilstein V, Cannone G, Graham S, Robinson CV, Spagnolo L, White MF. Structure of the CRISPR Interference Complex CSM reveals key similarities with Cascade. Molecular Cell 52:1, 124-134, 2013.
  • Schmidt C, Zhou M, Marriott H, Morgner N, Politis A, Robinson CV. Comparative crosslinking and mass spectrometry of an intact F-type ATPase suggest a role for phosphorylation in regulating subunit interactions and nucleotide occupancy. Nature Communications 4:1985,
  • Marcoux J, Wang S, Politis A, Reading E, Ma J, Biggins P, Zhou M, Tao H, Zhang Q, Chang G, Morgner N, Robinson CV. MS reveals synergistic binding of nucleotides, lipids and drugs to a multidrug resistance efflux pump. Proceedings of the National Academy of Sciences of the USA 110:24, 9704-9, 2013.
  • Politis A*, Park AY, Hall Z, Ruotolo BT, Robinson CV. Integrative modelling coupled with ion mobility MS reveals structural features of the clamp loader in complex with single-stranded DNA binding protein. Journal of Molecular Biology 425;4790-801, 2013.
  • Hall Z, Politis A*, Robinson CV. Structural modeling of heteromeric protein complexes from disassembly pathways and ion mobility-mass spectrometry. Structure 20, 1-14, 2012.
  • Hall Z, Politis A, Bush MF, Smith LJ, Robinson CV. Charge-state dependent compaction and dissociation of protein complexes: Insights from ion mobility and molecular dynamics. Journal of the American Chemical Society 134:3429-3438, 2012.
  • Zhou M, Morgner N, Barrera NP, Politis A, Isaacson SC, Matak-Vincovic D, Murata T, Stock D, Robinson CV. MS of intact V-type ATPase reveals bound lipids and the effects of nucleotide binding. Science 334:6054, 380-5, 2011.
  • Lane LA, Fernandez-Tornero C, Zhou M, Morgner N, Ptchelkine D, Steuerwald U, Politis A, Lindner D, Gvozdenovic J, Gavin AC, Müller CW, Robinson CV. Mass spectrometry reveals stable modules in holo and apo RNA polymerases I and II Structure 19, 90-100, 2011
  • Wang SC, Politis A, Di Bartolo N, Bavro VN, Tucker SJ, Booth PJ, Barrera NP, Robinson CV. Ion mobility-MS of two tetrameric membrane protein complexes reveals compact structures and differences in stability and packing. Journal of the American Chemical Society 132:15468-70, 2010.
  • Politis A, Park A, Hyung S, Barsky D, Ruotolo BT, Robinson CV. Integrating IM-MS with molecular modelling to determine the architecture of multiprotein complexes. PLoS ONE 5:e12080, 2010.
  • Park A, Jergic S, Politis A, Ruotolo, BT, Hirscberg D, Jesoop L, Beck J, Barsky D, O’Donell M, Dixon N, Robinson CV. A single subunit directs the assembly of the E. coli DNA sliding clamp loader. Structure 18,285-292, 2010.
  • Pukala TL, Ruotolo BT, Zhou M, Politis A, Stefanescu R, Leary JA, Robinson CV. Subunit architecture of multiprotein assemblies using restraints from gas-phase measurements. Structure 17, 1235-1243, 2009.
  • Politis A, Stavropoulos GP, Christolis MN, Panagopoulos PG, Vlachos NS, Markatos NC. Numerical modelling of simulated blood flow in idealized composite arterial coronary grafts: Transient flow. Journal of Biomechanics, 41, 1: 25-39. 2008.
  • Politis A, Stavropoulos GP, Christolis MN, Panagopoulos PG, Vlachos NS, Markatos NC. Numerical modelling of simulated blood flow in idealized composite arterial coronary grafts: Steady state simulations Journal of Biomechanics, 40, 5: 1115-1136. 2007.