The BSRC Fleming Flow Cytometry and Cell Sorting Facility is equipped with the spectral Cytek Aurora (5-laser/64-colour) analyzer and two conventional analyzers, BD FACS Canto II (2-laser/8-colour) and BD FACS Celesta (3-laser/12-colour) and a Luminex 200 Multiplex Analyzer. In addition, it is equipped with the spectral BD FACS Symphony S6 SE (5-laser/50-colour) and BD FACS Aria III (6-laser/18-colour) cell sorters, both operating under BSL-2 with enhanced precautions (during sorting operations).
The Facility provides investigators with support for flow cytometric analysis and operator-based cell sorting. Its personnel offer maintenance of the instruments, training and assistance on instrument usage and running of cell sorting experiments. Protocol design and establishment as well as troubleshooting and results analysis and interpretation are also provided depending upon the researcher's knowledge and requirements.
Flow cytometric analysis and cell sorting services are also available to external user.
The Facility also offers services for analysis of clinical-chemical and hematological parameters. It is equipped with Mindray BC5000Vet, a 5Diff Ηematology analyzer capable of mouse blood analysis and the Beckman Coulter AU480 Biochemical analyzer.
The Flow Cytometry and Cell Sorting facility is a key participant in the National Research Infrastructure InfrafrontierGR/Phenotypos.
BD FACS Symphony S6 SE
Spectral Cell Sorter
BD FACS Aria III
Cell Sorter
Cytek Aurora
Spectral Flow Cytometer
BD FACS Celesta
Flow Cytometer
BD FACS Canto II
Flow Cytometer
Luminex 200
Multiplex analyzer
Beckman Coulter AU480
Clinical Chemistry Analyzer
Mindray BC-5000Vet
Hematology Analyzer
The services provided include:
Flow Cytometry
- Multiparametric flow cytometric analysis
- Flow cytometric functional assay analysis (such as cell cycle, apoptosis and proliferation)
- 96/384 well plate high-throughput analysis
- Flow cytometer and Luminex multiplex bead-based immunoassays
Cell Sorting
- Isolation of Cellular subsets for functional assays
- Isolation of Cellular subsets for -omics analyses
- Single cell sorting
- Cell sorting screening assays
Clinical chemistry and Hematology
- Complete 5 population mouse blood count.
- Detection and quantification of common mouse serum/plasma and urine electrolytes, metabolites and enzymes.
Customized services
- Assays for the identification of cellular and molecular perturbations during phenotyping or disease modelling using cells in biological fluids (e.g. blood/sera) or tissues
- Establishment and optimization of customized cell sorting platforms
Services Request and Information
List of publications with data obtained at the Flow Cytometry and Cell Sorting Facility
Personnel
Sofia Grammenoudi, PhD
Staff Scientist A'
Facility Scientific and Operational Manager