The BSRC Fleming Flow Cytometry and Cell Sorting Facility is equipped with the BD FACS Canto II analyzer capable for up to 8-colour analysis and high-throughput applications and the BD FACS Celesta analyzer capable for up to 12-colour analysis as well as an 18-colour BD FACS Aria III cell-sorting unit operating under BSL-2 with enhanced precautions (during sorting operations).

The Facility provides investigators with support for flow cytometric analysis and operator-based cell sorting. Its personnel offer maintenance of the instruments, training and assistance on instrument usage and running of cell sorting experiments. Protocol design and establishment as well as troubleshooting and results analysis and interpretation are also provided depending upon the researcher's knowledge and requirements.

Flow cytometric analysis and cell sorting services are also available to external user.

The Facility also offers services for analysis of clinical-chemical and hematological parameters. It is equipped with Mindray BC5000Vet, a 5Diff Ηematology analyzer capable of mouse blood analysis and the Beckman Coulter AU480 Biochemical analyzer.

The Flow Cytometry and Cell Sorting facility is a key participant in the National Research Infrastructure InfrafrontierGR/Phenotypos.

Cell Sorter

BD FACS Canto II, BD FACS Celesta
Flow Cytometers

Beckman Coulter AU480
Clinical Chemistry Analyzer

Mindray BC-5000Vet
Hematology Analyzer

The services provided include:

Flow Cytometry

  • Multiparametric flow cytometric analysis
  • Flow cytometric functional assay analysis (such as cell cycle, apoptosis and proliferation)
  • Multiplex bead-based immunoassay analysis

Cell Sorting

  • Isolation of Cellular subsets for functional assays
  • Isolation of Cellular subsets for sequencing
  • Single cell sorting

Clinical chemistry and Hematology

  • Complete 5 population mouse blood count.
  • Detection and quantification of common mouse serum/plasma and urine electrolytes, metabolites and enzymes.

Customized services

  • Assays for the identification of cellular and molecular perturbations during phenotyping or disease modelling using cells in biological fluids (e.g. blood/sera) or tissues
  • Establishment and optimization of customized cell sorting platforms

Services Request and Information

Online Request Form


List of publications with data obtained at the Flow Cytometry and Cell Sorting Facility


Sofia Grammenoudi, PhD

Staff Scientist B'

Facility Scientific and Operational Manager

Kleopatra Dagla, MSc

Facility Technician and Instrument Operator