Our Facility generates live mice by performing surgical transfer of freshly collceted embryos. Embryo transfer has been demonstrated to be an effective method in order to reconstitute a pathogen-free mouse colony. The zona pellucida of embryos provides an effective barrier for pathogens.
This service can apply in the newly imported mice kept temporarily in the Quarantine as well as Fleming mice that need to get established in a different microbiological status (SOPF mice). The investigator provides a minimum amount of 3 male studs in reproductive age. According to the number of available male studs, an equal number of wild type females are superovulated and set up for mating. In case that the investigator requires mutated female mice to be used, he has to provide the required amount of females at the age of 4 weeks. The embryos are recovered at the 2-4 cell stage and transferred into pseudopregnant female mice.Upon births, we provide toe biopsies to the investigators for following screening. Another round is performed if no positive mice are produced in the first round.
Rederivation service order form
In case of request please fill out the FORM.