What we Do

The Transgenics & Gene Targeting Facility at the BSRC Al. Fleming was established in 2001 to integrate transgenic technologies in the mouse for cutting-edge biomedical research. We provide services for BSRC Al. Fleming investigators as well as for collaborating scientists in academic Institutes or biotech/pharmaceutical companies worldwide.

Interested parties are welcome to qo through our procedure pages and order forms. Inquires for competitive prices can be forwarded to This email address is being protected from spambots. You need JavaScript enabled to view it.

ES Cell Technology Services

Our services support the generation of mice carrying a disrupted or mutated specific genetic locus. To date, more than 50 projects have been completed successfully, including both obligatory and conditional knock-outs.

Generation of transgenic mice through:
Embryonic Stem Cell Gene Targeting, Blastocyst Injection, Chimera Production, Germline transmission.

DNA microinjection Service

Our services support the generation of transgenic mice carrying an inserted cassette in their genome. To date, more than 100 transgenic lines have been successfully generated.

Generation of transgenic mice through:
DNA microinjection in the pronucleus, generation of founders. The service is certified with ISO 9001:2000 since 2008

DNA microinjection

Archiving Services

Our Unit is member of the European Mouse Mutant Archive network since May 2010. With more than 350 lines deposited in our CryoBank we guarantee the proper maintenance and distribution of cryopreserved material.

Storage, archiving and transportation of mutant strains through
Embryo rederivation, cryopreservation and recovery of mouse embryo and sperm.
All services provided are in accordance with EMMA’s standards and protocols.

Personnel

Dimitris L. Kontoyiannis, Ph.D.

Scientist in Charge

Contact Info

Direct Tel Num:: (+30) 210.9656310
Email:: This email address is being protected from spambots. You need JavaScript enabled to view it.

Konstantinos Bozonelos, M.Sc

Operations Manager - EMMA Archive Manager

Contact Info

Direct Tel Num:: (+30) 210.9656310
Email:: This email address is being protected from spambots. You need JavaScript enabled to view it.

Christina Kosmopoulou

M.Sc CRISPR Specialist

Contact Info

Direct Tel Num:: (+30) 210.9656310
Email:: This email address is being protected from spambots. You need JavaScript enabled to view it.

Katerina Bavela

Transgenics specialist

Contact Info

Direct Tel Num:: (+30) 210.9656310
Email:: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vasileios Ntafis, DVM, PhD.

Supervising Veterinarian

Contact Info

Direct Tel Num:: (+30) 210.9656310
Email:: This email address is being protected from spambots. You need JavaScript enabled to view it.

Selected Publications

Trompouki E, Tsagaratou A, Kosmidis SK, Dollé P, Qian J, Kontoyiannis DL, Cardoso WV, Mosialos G. Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation. Neoplasia. (2009) May;11(5):469-76.

Panagopoulou P, Davos CH, Milner DJ, Varela E, Cameron J, Mann DL, Capetanaki Y. Desmin mediates TNF-alpha-induced aggregate formation and intercalated disk reorganization in heart failure. J Cell Biol. 2008 Jun 2;181(5):761-75.

Poulou M, Bell D, Bozonelos K, Alexiou M, Gavalas A, Lovell-Badge R, Remboutsika E. Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM “off-on” gene switch. PLoS One. 2010 Aug 31;5(8)

See all publications