Christoforos Nikolaou Research Group

Welcome to the Computational Genomics Group at BSRC "Alexander Fleming".

Computational Genomics Group (CG^2)

Our group was first established at the Biology Department of the University of Crete in 2010 and moved to BSRC “Alexander Fleming” in 2020. The group's expertise lies in the field of Computational Genomics. We are interested in questions related to chromatin structure, genome architecture and on how they may shape the regulation of gene expression in complex eukaryotic genomes. Towards this end, we are employing computational approaches in the analysis of -omics data, with particular focus on RNASeq, ChIPSeq, and Chromosome Conformation assays.


Starting from the early 2000s there has been an explosion of high-throughput genomics data enabling the analysis of complex biological phenomena at an unprecedented level of detail. The accumulation of such "-omics" data at multiple levels has allowed the dissection of transcriptomic complexity, the reconstruction of gene regulatory hierarchy and the apprehension of genome structure in three dimensions. Within this framework, our group has developed approaches to associate the local chromatin structure with gene expression and gene regulation networks. Our research program sees genomes as complex systems under the dual evolutionary constraints of structure and function.

The main objectives of our research focus on three major axes: 

1.The relationship between chromatin structure (from nucleosome positioning to chromatin states and large-scale chromosomal interactions) and the regulation of gene expression in homeostasis and disease. 

2.The role of genome structure in the rewiring of regulatory relationships during development, or disease progression. 

3.The evolution of genome architecture at various length scales.