The facility produces transgenic mice by microinjecting into the pronuclei of zygotes DNA construct provided by the investigator.
The submission of every DNA prep should be accompanied by: a) a proof that the transgene can be detected in genomic DNA by a functional assay (PCR or Southern blot). b) a gel photo where integrity and quantity of the DNA is documented with the help of a known DNA marker and a non-digested DNA sample. A total amount of at least 5 μgr DNA, is required. All documentation sent to the Facility should be provided electronically. If the DNA prep meets all requirements we will ask the investigator to proceed with the shipment. Upon receipt of DNA construct, it follows further purification of the construct, in order to make sure it will be microinjection-grade clean.
Female donor mice are superovulated and mated with fertile males. Zygotes are recovered and injected with purified DNA.
Injected zygotes are transferred into foster mothers. Following approximately 10 days after pups are born, we provide toe biopsies to the investigators for genotype screening.
The results of genotype screening and transgenic founder identification should be obtained ideally by weaning time, the latest when the mice are four weeks old. The minimum of 3 transgenic founders will be delivered for every construct injected.
DNA microinjection service form
In case of request please fill out the FORM.