Next Generation Sequencing (NGS) has revolutionized genomics, enabling a wide range of novel applications at a high throughput, genome-wide level. NGS enables massively parallel sequencing of nucleic acid fragments to address a variety of experimental questions, such as SNP profiling and mutation discovery, detection of structural variants, genome-wide measurement of mRNA transcript levels, alternative splicing events, microbiota compositions and a number of other applications.
The Fleming Genomics Facility was established in 2008. The Facility operates the MGI DNBSEQ-G400 and DNBSEQ-G99 Genetic Sequencers for next generation sequencing-based experimental protocols.
A broad variety of experimental protocols are supported by the Genomics Facility, such as whole genome sequencing, shotgun sequencing, targeted/exome sequencing, mRNA-seq, ribo-depleted RNA-seq, 3’ mRNA-seq, chromatin-targeted sequencing, spatial transcriptomics and single-cell sequencing, etc.
Libraries provided by users
Please contact Genomics Facility personnel prior to library construction. User-provided starting material and prepared libraries have to meet Genomics Facility QC standards before sequencing.
Quality & Amount of Starting material required per application
Please contact the Genomics Facility for precise instructions regarding the requirements in terms of quantity and quality of the provided DNA and/or RNA.
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