The Protein Chemistry facility provides a core facility for the analysis and characterization of proteins and their interactions with other biomolecules.

A Thermo Scientific LTQ Orbitrap XL hybrid FTMS is available since November 2010, providing an outstanding tool for advanced proteomics. It is equipped with a nanospray source and is suitable for quantitative proteomic analysis, employing SILAC or other isotope-labeling techniques. Peptides are separated with a specialized RSLCnano system (Ultimate 3000, Thermo). The Laboratory also has an LCQ-Deca ion trap (Thermo), equipped with standard electrospray, nanospray and a MALDI source. Data analysis is performed with Proteome Discoverer 1.3.

Another major instrument of the facility is a Surface Plasmon Resonance biosensor (BIAcore 3000) for the analysis of macromolecular interactions, an area where our group has extensive experience. All the necessary equipment for 2-D gels as well as a HPLC system and two FPLC systems for protein purification are also available.

Services

The Macromolecular Analysis Laboratory offers the following services:

  • Quantitative analysis of macromolecular interactions with the Biacore 3000 biosensor
  • Protein identification by nanospray mass-spectrometry
  • Identification of post-translational modifications
  • Separation of protein mixtures with two-dimensional electrophoresis
  • Separation of peptides by HPLC
  • Quantitative drug analysis
  • Protein expression and purification

Contact Information

For more information, please contact:

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Tel: +30-210-965-5054
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Tel: +30-210-9656310 ext.152

Please click here for the online LC - MS / MS ANALYSIS request form

B.S.R.C. "Alexander Fleming"
34 Fleming Street, 16672 Vari, Greece
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