Macromolecular Analysis Laboratory
Overview
The Macromolecular Analysis Laboratory provides a core facility for the
analysis and characterization of proteins and their interactions with other
biomolecules.
A Thermo Scientific LTQ Orbitrap XL hybrid FTMS is available since November
2010, providing an outstanding tool for advanced proteomics. It is equipped
with a nanospray source and is suitable for quantitative proteomic analysis,
employing SILAC or other isotope-labeling techniques. Peptides are separated
with a specialized nano-HPLC system (LC Packings UltiMate). The Laboratory
also has an LCQ-Deca ion trap (Thermo), equipped with standard electrospray,
nanospray and a MALDI source. Data analysis is performed with Proteome
Discoverer 1.2 (Thermo) enabling high-throughput quantitative proteomics.
Another major instrument of the facility is a Surface Plasmon Resonance
biosensor (BIAcore 3000) for the analysis of macromolecular interactions, an
area where our group has extensive experience. All the necessary equipment
for 2-D gels as well as a HPLC system and two FPLC systems for protein
purification are also available.
Services
The Macromolecular Analysis Laboratory offers the following services:
- Quantitative analysis of macromolecular interactions with the Biacore 3000 biosensor
- Protein identification by nanospray mass-spectrometry
- Identification of post-translational modifications
- Separation of protein mixtures with two-dimensional electrophoresis
- Separation of peptides by HPLC
- Quantitative drug analysis
- Protein expression and purification
Contact Information
For more information, please contact:
G. Panayotou
Tel: +30-210-965-5054M. Samiotaki
Tel: +30-210-9656310 ext.152
