Protein chemistry facility
Overview
The Laboratory of Protein Chemistry
provides a core facility for the
analysis and characterization of proteins and their interactions
with other biomolecules. A strong emphasis has been given on the
acquisition of state-of-the-art specialized instruments. A modern
ion-trap mass spectrometer is available (Finnigan LCQ-DECA),
providing high sensitivity and accuracy. Apart from the standard
electrospray source, we have obtained a nanospray source, which is
essential for the analysis of peptides at the low levels obtained
from 2-D gels. Peptides are separated with a specialized nano-HPLC
system (LC Packings UltiMate), using flow rates at the nanoliter
range and very small diameter columns. All the necessary equipment
for reproducible 2-D gels are available. A major instrument of the
facility is a Surface Plasmon Resonance biosensor (BIAcore 3000) for
the analysis of macromolecular interactions, an area where our group
has extensive experience. An analytical HPLC system, as well as two
FPLC systems for protein purification are also available.
Services
The Protein Chemistry facility offers the following services:
- Quantitative analysis of macromolecular interactions with the Biacore 3000 biosensor
- Protein identification by nanospray mass-spectrometry
- Identification of post-translational modifications
- Separation of protein mixtures with two-dimensional electrophoresis
- Separation of peptides by HPLC
- Quantitative drug analysis
- Protein expression and purification
Contact Information
For more information, please contact:
G. Panayotou
Tel: +30-210-965-5054
