Flow cytometry facility // Al. Fleming BSRC

RESOURCES-ANALYSERS

• BD FACS Canto II, analyser, available to internal trained users.

Two Excitation Sources
Coherent Sapphire air-cooled laser, 488nm (green), 20mW
JDS Uniphase Helium/Neon, air-cooled laser, 633nm (red), 20mW

Ten Channels
2 light scatter channels (FSC, SSC)
8 fluorescent channels (FL1-FL8)

Software
BD FACS DivaÔ v 5.0.1

Options
BD High Throughput Sampler (HTS) for automated microtiter plate acquisition

• Beckman Coulter® Epics® XL-MCL, analyser, restricted users only

Single Excitation Source
Air-cooled Argon ion laser, 488nm-15mW

Six Channels
2 light scatter channels (FSC, SSC)
4 fluorescent channels (FL1-FL4)

RESOURCES-SORTER

• BD FACS Vantage SE II, cell sorter, available as an internal service

Single Excitation Source
Coherent® Enterprise IIC water-cooled laser, 488nm-200mW and UV (351-364nm)-50mW

Six Channels
2 light scatter channels (FSC, SSC)
4 fluorescent channels (FL1-FL4)

Software
BD CellQuest

Current Sorting Options
Standard: sorting speed of 3000 cells/sec

Future Options
MacroSort Plus: allows sorting of large cells
TurboSort Plus: allows sorting at high speeds, 25000 cells/sec
CloneCyt Plus: allows sorting in multiwell plates