Ερευνητική ομάδα Γιώργου Παναγιώτου

Δημοσιεύσεις

2005

Saridaki A.,  Panayotou G.,  2005, "Identification of growth factor-regulated proteins using 2D electrophresis and mass spectrometry", Growth Factors, (in press).

2004

Nemazanyy I., Panasyuk G., Zhyvoloup A., Panayotou G., Gout I. T., Filonenko V., 2004, "Specific interaction between S6K1 and CoA synthase: a potential link between the mTOR/S6K pathway, CoA biosynthesis and energy metabolism", FEBS Lett., 578, 357-362 [PubMed].

Panayotou G., 2004, "Molecular analysis of signal transduction pathways", Autoimmun. Rev., 3, S28-9 [PubMed].

Sidera K., Samiotaki M., Yfanti E., Panayotou G., Patsavoudi E., 2004, "Involvement of cell surface HSP90 in cell migration reveals a novel role in the developing nervous system", J. Biol. Chem., 279, 45379-45388 [PubMed].

Foukas L. C., Panayotou G., Shepherd P. R., 2004, "Direct interaction of MHC class II derived peptides with class Ia PI 3-kinase results in dose-dependent stimulatory effects", J. Biol. Chem., 279, 7505-7511 [PubMed].

2003

Vardakou M., Katapodis P., Samiotaki M., Kekos D., Panayotou G., Christakopoulos P., 2003, "Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan", Int. J. Biol. Macromol., 33, 129-34 [PubMed].

Dekker S., Toussaint W., Panayotou G., de Wit T., Visser P., Grosveld F., Drabek D., 2003, "Intracellularly expressed single domain antibody prevents the production of porcine retroviruses", J. Virol., 77, 12132-12139 [PubMed].

Staikou E. V., Routsias J. G., Makri A. A., Terzoglou A., Sakarellos-Daitsiotis M., Sakarellos C., Panayotou G., Moutsopoulos H. M., Tzioufas A. G., 2003, "Calreticulin binds preferentially with B cell linear epitopes of Ro60 kD autoantigen, enhancing recognition by anti-Ro60 kD autoantibodies", Clin. Exp. Immunol., 134, 143-50 [PubMed].

Zamanou A., Samiotaki M., Panayotou G., Margaritis L., Lymberi P., 2003, "Fine specificity and subclasses of IgG anti-actin autoantibodies differ in health and disease", J. Autoimm., 20, 333-334 [PubMed].

2002

Gonos E. S., Agrafiotis D., Dontas A. S., Efthimiopoulos S., Galaris D., Karamanos N. K., Kletsas D., Kolettas E., Panayotou G., Pratsinis H., Sekeri-Pataryas K. E., Simoes D., Sourlingas T. G., Stathakos D., Stratigos A. J., Tavernarakis N., Trougakos I. P., Tsiganos C. P., Vynios D. H., 2002, "Ageing research in Greece", Exp. Gerontol., 37 (6), 735-747 [PubMed].

Chan T. O., Rodeck U., Chan A. M., Kimmelman A. C., Rittenhouse S. E., Panayotou G., Tsichlis P. N., 2002, "Small GTPases and tyrosine kinases coregulate a molecular switch in the phosphoinositide 3-kinase regulatory subunit", Cancer Cell, 1, 181-191 [PubMed].

Theodosiou A. ,and Ashworth A., 2002, "MAP kinase phosphatases", Genome Biol., 3(7) , 3009 (1-10) [PubMed].

2001

Panayotou G., Ladbury J., 2001, "Analysis of SH2 domain - phosphopeptide interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance", Methods Mol. Biol., 124, 295-311 [PubMed].

2000

Rosler A., Panayotou G., Hornby D. P., Barlow T., Brown T., Pearl L. H., Savva R., Blackburn G. M., 2000, "The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues", Nucleosides Nucleotides Nucleic Acids, 19, 1505-1516 [PubMed].

Baynes K. C., Beeton C. A., Panayotou G., Stein R., Soos M., Hansen T., Simpson H., O'Rahilly S., Shepherd P. R., Whitehead J. P., 2000, "Natural variants of human p85 alpha phosphoinositide 3-kinase in severe insulin resistance: a novel variant with impaired insulin-stimulated lipid kinase activity", Diabetologia, 43, 321-331 [PubMed].

1999

Greagg M. A., Fogg M. J., Panayotou G., Evans S. J., Connolly B. A., Pearl L. H., 1999, "A read-ahead function in archaeal DNA polymerases detects pro-mutagenic template-strand uracil", Proc. Natl. Acad. Sci. U.S.A., 96, 9045-9050 [PubMed].

Chaudri Z. N., Barlet-Jones M., Panayotou G., Klonisch T., Roitt I. M., Lund T., Delves P. J., 1999, "Dual specificity antibodies using a double-stranded oligonucleotide bridge", FEBS Lett., 450, 23-26 [PubMed].

Lo Surdo P., Bottomley M. J., Arcaro A., Siegal G., Panayotou G., Sankar A., Gaffney P. R. J., Riley A. M., Potter B. V. L., Waterfield M. D., Driscoll P. C., 1999, "Structural and biochemical evaluation of the interaction of the phosphatidylinositol 3-kinase p85α SH2 domains with phosphoinositides and inositol polyphosphates", J. Biol. Chem., 274, 15678-15685 [PubMed].

Harpur A. G., Layton M. J., Das P., Bottomley M. J., Panayotou G., Driscoll P. C., Waterfield M. D., 1999, "Intermolecular interactions of the p85 a regulatory subunit of phosphatidylinositol 3-kinase", J. Biol. Chem., 274, 12323-12332 [PubMed].

1998

Keusch J., Panayotou G., Malissard M., Berger E. G., Appert H. E., Lydyard P. M., Delves P. J., 1998, "Antibody recognition of epitopes on wild-type and mutant β-(1>4)-galactosyltransferase-1", Carbohydrate Res., 313, 37-48 [PubMed].

Bottomley M. J., Salim K., Panayotou G., 1998, "Phospholipid-binding protein domains", Biochim. Biophys. Acta, 1436, 165-183 [PubMed].

Layton M. J., Harpur A. G., Panayotou G., Bastiens P. I. H., Waterfield M. D., 1998, "Binding of a diphosphotyrosine-containing peptide that mimics activated Platelet-derived Growth Factor Receptor β induces oligomerisation of phosphatidylinositol 3-kinase", J. Biol. Chem., 273, 33379-33385 [PubMed].

Panayotou G., 1998, "Surface Plasmon Resonance: Measuring protein interactions in real time", Methods Mol. Biol., 88, 1-10 [PubMed].

Siegal G., Davis B., Kristensen S. M., Sankar A., Linacre J., Stein R., Panayotou G., Waterfield M. D., Driscoll P. C., 1998, "Solution structure of the C-terminal SH2 domain of the p85α regulatory subunit of phosphoinositide 3-kinase", J. Mol. Biol., 276, 461-478 [PubMed].

Barrett T. E., Savva R., Panayotou G., Barlow T., Brown T. A., Jiricny J., Pearl L. H., 1998, "Crystal structure of a G:T/U mismatch-specific DNA glycosylase: Mismatch recognition by complementary-strand interactions", Cell, 92, 117-129 [PubMed].

Flynn P., Mellor H., Palmer R., Panayotou G., Parker P. J., 1998, "Multiple interactions of PRK1 with RhoA. Functional assignment of the HR1 repeat motif", J. Biol. Chem., 273, 2698-2705 [PubMed].

Panayotou G., Brown T., Barlow T., Pearl L. T., Savva R., 1998, "Direct measurement of the substrate preference of Uracil-DNA Glycosylase", J. Biol. Chem., 273, 45-50 [PubMed].

Panayotou G., 1998, "Surface Plasmon Resonance", In 'Encyclopedia of Immunology' (2nd edition; eds. Delves and Roitt), Academic Press, 2247-2250.

1997

Vanhaesebroeck B., Leevers S. J., Panayotou G., Waterfield M. D., 1997, "Phosphoinositide 3-kinases: a conserved family of signal transducers", Trends Biochem. Sci., 22, 267-272 [PubMed].

Reith A. D., Panayotou G., 1997, "Growth factor signalling pathways in cancer", In 'Cellular and Molecular Biology of Cancer', (eds. Franks and Teich), 3rd edition, Oxford University Press, 230-250.

1996

Salim K., Bottomley M. J., Querfurth E., Zvelebil M., Gout I., Scaife R., Margolis R. L., Gigg R., Smith C. I. E., Driscoll P. C., Waterfield M. D., Panayotou G., 1996, "Distinct specificity in the recognition of phosphoinositides by the Pleckstrin Homology domains of dynamin and Bruton's tyrosine kinase (Btk)", EMBO J., 15, 6241-6250 [PubMed].

Klonisch T., Panayotou G., Edwards P., Jackson A. M., Berger P., Delves P. J., Lund T., Roitt I. M., 1996, "Enhancement in antigen binding by a combination of synergy and antibody capture", Immunology, 89, 165-171 [PubMed].

Spanopoulou E., Zaitseva F., Wang F-H., Santagata S., Baltimore D., Panayotou G., 1996, "The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination", Cell, 87, 263-276 [PubMed].

Ladbury J..E., Hensmann M., Panayotou G., Campbell I. D., 1996, "Alternative modes of tyrosyl phosphopeptide binding to a Src family SH2 domain: Implications for regulation of tyrosine kinase activity", Biochemistry, 35, 11062-11069 [PubMed].

Klonisch T., Delves P., Berger P., Panayotou G., Lapthorn A. J., Isaacs N. W., Wick G., Lund T., Roitt I. M., 1996, "Relative location of epitopes involved in synergistic antibody binding using human chorionic gonadotropin as a model", Eur. J. Immunol., 26, 1897-1905 [PubMed].

Wientjes F. B., Panayotou G., Reeves E., Segal A. W., 1996, "Interactions between cytosolic components of the NADPH oxidase-p40phox interacts with both p67phox and p47phox", Biochem. J., 317, 919-924 [PubMed].

Wymann M. P., Bulgarelli-Leva G., Zvelebil M. J., Pirola L., Vanhaesebroeck B., Waterfield M. D., Panayotou G., 1996, "Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction", Mol. Cell. Biol., 16, 1722-1733 [PubMed].

Zvelebil M. J., MacDougall L., Leevers S., Volinia S., Vanhaesebroeck B., Gout I., Panayotou G., Domin J., Stein R., Pages F., Koga H., Salim K., Linacre J., Das P., Panaretou C., Wetzker R., Waterfield M. D., 1996, "Structural and functional diversity of phosphoinositide 3-kinases", Phil. Trans. R. Soc. Lond., 351, 217-223 [PubMed].

Ellis C., Kasmi F., Ganju P., Walls E., Panayotou G., Reith A. D., 1996, "A juxtamembrane autophosphorylation site in the Eph family receptor tyrosine kinase, Sek, mediates high affinity interaction with p59fyn", Oncogene, 12, 1727-1736 [PubMed].

Panayotou G., 1996, "Multiple roles for PI 3-kinases in signal transduction", In 'Signal Transduction' (eds. Heldin C.-H. and Purton M.), Chapman and Hall, 189-204.

1995

Zvelebil M., Panayotou G., Waterfield M. D., 1995, "Prediction and analysis of SH2 domain - phosphopeptide interactions", Protein Engineering, 8, 527-533 [PubMed].

Pelicci G., Giordano S., Zhen Z., Salcini A. E., Lanfrancone L., Bardelli A., Panayotou G., Waterfield M. D., Ponzetto C., Pelicci P. G., Comoglio P. M., 1995, "The motogenic and mitogenic responses to HGF are amplified by the Shc adaptor protein", Oncogene, 10, 1631-1638 [PubMed].

1994

Dhand R., Hiles I., Panayotou G., Fry M. J., Roche S., Gout I., Totty N. F., Truong O., Yonezawa K., Kasuga M., Courtneidge S., Waterfield M. D., 1994, "PI 3-kinase is a dual specificity kinase: Autoregulation by an intrinsic protein-serine kinase activity", EMBO J., 13, 522-533 [PubMed].

Dhand R., Hara K., Hiles I., Bax B., Gout I., Panayotou G., Fry M. J., Yonezawa K., Kasuga M., Waterfield M. D., 1994, "PI 3-kinase: Structural and functional analysis of intersubunit interactions", EMBO J., 13, 511-521 [PubMed].

Hensmann M., Booker G. W., Panayotou G., Boyd J., Linacre J., Waterfield M. D., Campbell I. D., 1994, "Phosphopeptide binding to the N-terminal SH2 domain of the p85α subunit of PI 3-kinase: A heteronuclear NMR study", Protein Science, 3, 1020-1030 [PubMed].

Ponzetto C., Bardelli A., Zhen Z., della Zonca P., Maina F., Giordano S., Graziani A., Panayotou G., Comoglio P. M., 1994, "A multifunctional docking site mediates signalling and transformation by the hepatocyte growth factor / scatter factor (HGF/SF) receptor family", Cell, 77, 261-271 [PubMed].

1993

Panayotou G., Waterfield M. D., End P., 1993, "Riding the evanescent wave", Current Biol., 3, 913-915 [PubMed].

Gout I., Dhand R., Hiles I. D., Fry M. J., Panayotou G., Das P., Truong O., Totty N. F., Hsuan J., Booker G. W., Campbell I. D., Waterfield M. D., 1993, "The GTPase dynamin binds to and is activated by a subset of SH3 domains", Cell, 75, 25-36 [PubMed].

Panayotou G., Gish G., End P., Truong O., Gout I., Dhand R., Fry M. J., Hiles I., Pawson T., Waterfield M. D., 1993, "Interactions between SH2 domains and tyrosine-phosphorylated PDGF β-receptor sequences: Analysis of kinetic parameters using a novel biosensor-based approach", Mol. Cell. Biol., 13, 3567-3576 [PubMed].

Panayotou G., Waterfield M. D., 1993, "The assembly of signalling complexes by activated growth factor receptors", BioEssays, 15, 171-177 [PubMed].

Ruiz-Larrea F., Vicendo P., Yaish P., End P., Panayotou G., Fry M. J., Morgan S. J., Thompson A., Parker P. J., Waterfield M. D., 1993, "Characterisation of the bovine brain cytosolic phosphatidylinositol 3-kinase complex", Biochem. J., 290, 609-616 [PubMed].

Fry M. J., Panayotou G., Booker G. W., Waterfield M. D., 1993, "New insights into protein-tyrosine kinase receptor signalling complexes", Protein Science, 2, 1785-1797 [PubMed].

Ponzetto C., Bardelli A., Maina F., Longati P., Panayotou G., Dhand R., Waterfield M. D., Comoglio P. M., 1993, "A novel recognition motif for phosphatidylinositol 3-kinase binding mediates its association with the Hepatocyte Growth Factor / Scatter Factor receptor", Mol. Cell. Biol., 13, 4600-4608 [PubMed].

End P., Gout I., Fry M. J., Panayotou G., Dhand R., Yonezawa K., Kasuga M., Waterfield M. D., 1993, "A biosensor approach to probe the structure and function of the p85α subunit of the PI 3-kinase complex", J. Biol. Chem., 268, 10066-10075 [PubMed].

White H., Totty N., Panayotou G., 1993, "Haemonectin, a granulocytic cell binding protein, is highly related to the plasma glycoprotein fetuin", Eur. J. Biochem., 213, 523-528 [PubMed].

1992

Panayotou G., Waterfield M. D., 1992, "Phosphatidylinositol 3-kinase: a key enzyme in diverse signalling processes", Trends Cell Biol., 2, 358-360 [PubMed].

Panayotou G., Fry M. J., Waterfield M. D., 1992, "Multi-enzyme complexes associated with receptors during signal transduction", C. R. Acad. Sci. Paris, 314, 11-20 [PubMed].

End P., Panayotou G., Entwistle A., Waterfield M. D., Chiquet M., 1992, "Tenascin: a modulator of cell growth", Eur. J. Biochem., 209, 1041-1051 [PubMed].

McGlade C. J., Ellis C., Reedijk M., Anderson D., Mbamalu G., Reith A. D., Panayotou G., End P. Bernstein A., Kazlauskas A., Waterfield M. D., Pawson T., 1992, "SH2 domains of the p85α subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors", Mol. Cell. Biol., 12, 991-997 [PubMed].

Goot I., Dhand R., Panayotou G., Fry M. J., Waterfield M. D., 1992, "Expression and characterisation of two 85 kDa components of the PI 3-kinase complex using baculovirus vectors", Biochem. J., 288, 395-405 [PubMed].

Panayotou G., Bax B., Gout I., Federwisch M., Wroblowski B., Dhand R., Fry M. J., Blundell T. L., Wollmer A., Waterfield M. D., 1992, "Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: Structural features and analysis of conformational changes", EMBO J., 11, 4261-4272 [PubMed].

Booker G. W., Breeze A. L., Downing A. K., Panayotou G., Gout I., Waterfield M. D., Campbell I. D., 1992, "Structure of an SH2 domain of the p85α subunit of phosphatidylinositol 3-kinase", Nature, 358, 684-687 [PubMed].

Hiles I. D., Otsu M., Volinia S., Fry M. J., Gout I., Dhand R., Panayotou G., Ruiz-Larrea F., Thompson A., Totty N. F., Hsuan J. J., Courtneidge S. A., Parker P. J., Waterfield M. D., 1992, "Phosphatidylinositol 3-kinase: Structure and expression of the 110 kd catalytic subunit", Cell, 70, 419-429 [PubMed].

Fry M. J., Panayotou G., Ruiz-Larrea F., Waterfield M. D., 1992, "Purification and charaterisation of the binding specificity of the PtdIns 3-kinase complex of bovine brain using phosphopeptide affinity columns", Biochem. J., 288, 383-393 [PubMed].

1991

Otsu M., Hiles I., Gout I., Fry M. J., Ruiz-Larrea F., Panayotou G., Thompson A., Dhand R., Hsuan J., Totty N., Smith A. D., Morgan S., Courtneidge S. A., Parker P., Waterfield M. D., 1991, "Characterization of two 85 kDa proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase", Cell, 65, 91-104 [PubMed].

Khazaie K., Panayotou G., Aguzzi A., Samarut J., Gazzolo L., Jurdic P., 1991, "EGF promotes in vivo tumorigenic growth of primary chicken embryo fibroblasts expressing v-myc and enhances in vitro transformation by the v-erbA oncogene", Oncogene, 6, 21-28 [PubMed].

Fry M. J., Panayotou G., Waterfield M. D., 1991, "Oncogenes and their relation to growth regulating receptors", In 'Receptor Sub-units and Complexes' (eds. Bergen A. S. V., Roberts G. C. K.), Cambridge University Press, 15.

1990

Panayotou G., Haley J. D., Hsuan J. J., Clark S., Waterfield M. D., 1990, "The structure and function of growth factor receptors and their subversion in cancer", In 'New Concepts in Cancer: Metastasis, Oncogenes and Growth Factors' (eds. C. Etievant, J. Cros and Y. M.Rustum - Macmillan Press, London), 166-180.

Panayotou G., Gregoriou M., 1990, "Purification of the epidermal growth factor receptor from A431 cells", In 'Receptor Biochemistry, A Practical Approach' (ed. E. Hulme), IRL Press, Oxford/Washington DC, 203-211.

1989

Panayotou G., Waterfield M. D., 1989, "Cell surface receptors for polypeptide hormones, growth factors and neuropeptides", Current Opin. Cell Biol., 1, 167-176 [PubMed].

Panayotou G., End P., Aumaily M., Timpl R., Engel J., 1989, "Domains of laminin with growth factor activity", Cell, 56, 93-101 [PubMed].

Hsuan J. J., Panayotou G., Waterfield M. D., 1989, "Structural basis for epidermal growth factor receptor function", In 'Progress in Growth Factor Research' (ed. J. K. Heath), 1, 23-32 [PubMed].

Panayotou G., Hsuan J. J., Waterfield M. D., 1989, "Ligand- and antibody-affinity purification of the EGF receptor", In 'Receptor Purification' (ed. G. Litwack - Humana Press, New Jersey), 1, 289-302.

1988

Farzaneh F., Panayotou G. N., Bowler L. D., Hardas B. D., Broom T., Walther C., Shall,S., 1988, "ADP-ribosylation is involved in the integration of foreign DNA into the mammalian cell genome", Nucleic. Acids. Res., 16, 11319-11326 [PubMed].

Pallen C. J., Sahlin L., Panayotou G., Waterfield M. D., 1988, "Purification and characterization of placental membrane phosphotyrosine phosphatases", Cold Spring Harbor Symp. Quant. Biol., 53, 447-454 [PubMed].

Pallen C. J., Panayotou G. N., Sahlin L., Waterfield M. D., 1988, "Purification of a phosphotyrosine phosphatase that dephosphorylates the epidermal growth factor receptor autophosphorylation sites", Ann. N.Y. Acad. Sci., 551, 299-308 [PubMed].

1986

Panayotou G., Magee A., Gregoriou M., Rees A., Geisow M. J., 1986, "Transfer of reconstituted EGF receptor to receptor-negative cells by liposome fusion", Trans. Biochem. Soc., 14, 298-299.

1985

Panayotou G. N., Magee A. I., Geisow M. J., 1985, "Reconstitution of the epidermal growth factor receptor in artificial lipid bilayers", FEBS Lett., 183, 321-325.

1984

Geisow M. J., Childs J., Dash B., Harris A., Panayotou G., Sudhof T., Walker J. H., 1984, "Cellular distribution of three mammalian calcium-binding proteins related to Torpedo calelectrin", EMBO J., 3, 2969-2974 [PubMed].

Shall S., Creissen D., Farzaneh F., Irwin J., Murray B., Panayotou G., Tavassoli M., Tavassoli M., 1984, "ADP-Ribose in DNA repair and carcinogenesis", In 'Genes and Cancer' (eds. Bishop J. M., Rowley J. D., Greaves M. - Liss, New York), 175-183.